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The specific field of research for this Research Scientist Assistant - SBC is in biophysical characterization using x-ray crystallography, including crystallization, diffraction, and solving of structures for peptides and proteins. Potentially assistance with electron microscopy and high resolution microscopy.
The Department of Biochemistry at the University of Washington has contributed to many phases of research. Initially recognized for incisive studies of the structure and function of proteins, and the mechanisms of enzyme action, the department branched out into a multitude of other areas. These included: developmental biology of flies, fish, frogs, and mice; molecular mechanisms of aging in yeast and mammals; vertebrate visual and immune responses; chromosome dynamics, cell division, DNA replication and repair; energy transduction during photosynthesis; protein secretion and membrane fusion; translational control and regulated protein degradation; protein structure determination by crystallography and NMR spectroscopy; and protein structure prediction and enzyme design. Our diversity of interests guarantees a rich intellectual and educational environment.
About the Institute for Protein Design:
The Institute for Protein Design (IPD) at the University of Washington in Seattle is creating a new world of synthetic proteins to address 21st-century challenges in medicine, energy, and technology. The IPD is a unique interdisciplinary environment where world-class researchers with expertise in computing and software, biochemistry, genome sciences, biological structure, pharmacology, immunology and other basic science disciplines, as well as clinical medicine, work together to both computationally design and experimentally characterize novel biomolecules. Current projects include the design of novel therapeutics for cancer, neurological diseases, and more, as well as new vaccines, nanomaterials, and enzymes.
The UW Institute for Protein Design currently has an outstanding opportunity for a Research Scientist Assistant –SBC to support peptide and protein crystallography efforts for the IPD. The Research Scientist Assistant - SBC will be assigned work under general guidance and receive instruction on specific assignment objectives, complex features, and possible solutions. Guidance on unusual problems will be provided to the Research Scientist Assistant - SBC by IPD Faculty, other UW Faculty, or other experts in biophysical characterization and work shall be reviewed for application of sound professional judgment. .
The Research Scientist Assistant - SBC will interface directly with members of other IPD Core Laboratories as well as graduate students and postdoctoral fellows in the laboratories of IPD Faculty. The research activities will involve setup of crystallization trays, screening for crystal formation and growth using microscopy, operation of X-ray generation sources, and eventually training in data collection and solving structures. The RSA SBC will grow to be an expert in protein and peptide x-ray crystallography and will therefore be of assistance in optimization of crystallization conditions and support of crystallography needs for the IPD. With the gaining of expertise, high throughput robotics, maintenance and operation of x ray sources and independent operation in crystallography projects.
In this role, ~40% of the effort will be dedicated to the setup and monitoring of crystallography trays.
Another ~20% of effort will be dedicated to maintenance and operation of liquid handling robotics.
The remaining ~40% of effort will, eventually, be dedicated to the maintenance and operation of x-ray generating sources for x-ray diffraction of peptides and proteins.
Bachelors's Degree in Biochemistry, Biology or similar + less than 3 months of experience in related field.
The Research Scientist Assistant-SBC is devoted primarily to scientific research and requires a B. Sc. in a biology-related field, with less than 3 months of experience in characterization of protein. Candidates must be capable of applying standardized scientific procedures and techniques to assignments of limited complexity, involving potentially conflicting design requirements.
An ideal Research Scientist Assistant - SBC candidate will be someone who (i) has had experience in a laboratory setting during the undergraduate degree obtention, (ii) will use their knowledge gained in their undergraduate degree program to apply fundamental concepts, practices and procedures, (iii) follows procedures for small projects and/or major project tasks which may last years under immediate supervision, and effectively manages them under guidance and direction from project staff; interfaces with investigator peers, (iv) can provide timely and professional scientific services to a number of internal collaborators on approximately 2-3 different projects, (v) can perform tasks and methods as directed, (vi) and enjoys interacting with other scientists in a highly collaborative and fast-paced work environment.
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